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BrainStream provides an open-source, MATLAB-based environment for definition and execution of BCI experiments. Key concept is that incoming markers in the data stream dynamically determine the actions of the system. Users can predefine a user variable space and a variety of actions for incoming markers, each with different options for the exact timing of execution. BrainStream integrates well with popular MATLAB-based analysis toolboxes, such as Fieldtrip (http://www.ru.nl/neuroimaging/fieldtrip