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Methods of 16S sequencing data analysis have evolved and changed rapidly over the past few years, making most available online tutorials for QIIME out of date with respect to either sequencing technology, QIIME syntax and scripts, or best practices in statistical analysis. On top of that, the sheer number of scripts and methods packaged with QIIME (or other microbial analysis pipelines!) might be overwhelming to someone new to the field. This page intends to be an accessible and straightforward